Osteopontin Expression in Polarized MDCK Cells
نویسندگان
چکیده
منابع مشابه
Polarized exocytosis in MDCK cells is regulated by phosphorylation.
Protein phosphorylation and dephosphorylation systems modulate many cellular activities and have recently been implicated in the in vitro transport of newly synthesized proteins. Here we show that polarized transport from the Golgi to the plasma membrane in intact MDCK cells is regulated by phosphorylation-dephosphorylation. Transport is inhibited by the phosphatase inhibitor okadaic acid and i...
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The release of nucleotides is involved in mechanosensation in various epithelial cells. Intriguingly, kidney epithelial cells are absolutely dependent on the primary cilium to sense changes in apical laminar flow. During fluid passage, the renal epithelial cells are subjected to various mechanical stimuli in addition to changes in the laminar flow rate. In the distal part of the collecting duct...
متن کاملIntegrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity.
Epithelial cells polarize in response to contacts with the extracellular matrix and with neighboring cells. Interactions of cells with the extracellular matrix are mediated mainly by the integrin family of receptors. To begin to understand the role of integrins in polarization, we have investigated the expression and localization of three integrin families in the polarized Madin-Darby canine ki...
متن کاملExpression of caveolin-1 and polarized formation of invaginated caveolae in Caco-2 and MDCK II cells.
We have studied caveolin-1 expression and the frequency and distribution of typical invaginated caveolae as they are identified by electron microscopy in the polarized epithelial cell lines MDCK II and Caco-2. In wild-type MDCK II cells caveolin expression is high and more than 400 caveolae/mm filter were observed at the basolateral membrane. No caveolae were found at the apical surface. By con...
متن کاملFAPP2 is involved in the transport of apical cargo in polarized MDCK cells
Phosphatidylinositol-4-phosphate (PI(4)P) is the main phosphoinositide in the Golgi complex and has been reported to play a pleiotropic role in transport of cargo from the trans-Golgi network to the plasma membrane (PM) in polarized Madin-Darby canine kidney (MDCK) cells. Overexpression of the chimeric fluorescent protein encoding the pleckstrin homology domain, which is specific for PI(4)P, in...
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ژورنال
عنوان ژورنال: Kafkas Universitesi Veteriner Fakultesi Dergisi
سال: 2014
ISSN: 1300-6045
DOI: 10.9775/kvfd.2014.10815